The detection of progesterone is mainly carried out through blood sampling, and the specific detection methods may include liquid chromatography and other methods.

Here are the general steps and considerations for progesterone testing:

Preparations before Testing: Before testing progesterone, it is necessary to clarify the purpose of the test. Different testing purposes, such as understanding ovarian function, corpus luteum function, or progesterone levels in early pregnancy, can affect the timing and frequency of testing. Additionally, it is recommended to keep fasting before blood sampling to avoid potential interference from food intake on the test results.

Blood Sampling Process:
The preferred blood sampling site is the elbow vein, where the patient needs to expose the arm with more prominent veins, and if necessary, remove outer clothing.
The doctor will tie a tourniquet above the puncture site and disinfect the local skin.
During blood sampling, the patient should clench their fist to allow better venous filling. There may be a slight sensation of pain when the needle is inserted and removed. If feeling anxious, the patient can distract their attention, such as counting silently "1, 2, 3," to reduce tension.
After blood collection, the patient should release their fist and press a cotton ball with their index or middle finger at the puncture site for 3 to 5 minutes to stop the bleeding. If there is a tendency for bleeding, the compression should be extended for 5 to 10 minutes.

Testing Method: Progesterone testing is primarily conducted through methods such as liquid chromatography. The doctor will select a standard working solution with a similar concentration based on the progesterone content in the sample solution, and determine the progesterone level by comparing the response values of the standard working solution and the sample solution.

Result Interpretation: The progesterone test results need to be interpreted with reference to the normal progesterone values for different periods. During non-pregnancy, progesterone levels vary during different phases of the menstrual cycle, such as the follicular phase, luteal phase, and menopause, with normal values ranging from 0.6-1.9nmol/l, 20.7-102.4nmol/l, and 0-3.2nmol/l, respectively. After pregnancy, progesterone levels gradually increase with the progression of gestational weeks, and the specific values vary according to the week of pregnancy. If progesterone levels are below the normal range, it may lead to issues such as miscarriage and requires prompt medical attention.

The above information on the general steps and considerations for progesterone testing is for reference only. Please consult a professional doctor for specific needs.