The results of dog breeding detection can cover multiple aspects, including the judgment of estrus cycle, determination of ovulation time, and detection of pregnancy.

Here is a clear answer regarding the results of dog breeding detection based on reference articles:

I. Judging the Estrus Cycle

The estrus cycle of female dogs: Normally, female dogs go into estrus twice a year, in spring (March to May) and autumn (September to November). Each estrus lasts for 5 to 12 days, averaging 9 days, with a few cases lasting up to 21 days.

Manifestations during estrus: Female dogs exhibit increased excitability, activity, restlessness, loud barking, bright eyes, and other symptoms during estrus. At the same time, the vulva swells, reddens, and discharges red mucus mixed with blood, leading to a decrease in appetite.

II. Determining the Time of Ovulation

Progesterone testing: Progesterone testing is currently the most advanced and accurate method to determine the ovulation cycle of female dogs. By detecting changes in progesterone levels in the blood, the optimal breeding period can be identified, improving the female dog's pregnancy rate and litter size.

Vaginal smear method: The ovulation period is judged by observing changes in the electrical resistivity or cell keratinization characteristics of canine vaginal mucus. However, a single smear test may provide misleading results, so multiple smears and systematic evaluation are recommended.

Ovulation meter inspection: The ovulation meter is a device that measures changes in the electrical resistivity of canine vaginal mucus. According to the instructions, the best time for breeding is generally 1-2 days after ovulation.

LH (Luteinizing Hormone) peak detection: The LH peak usually appears 3-12 days after observing estrus symptoms, and ovulation occurs 2-3 days after the LH peak. Therefore, detecting the LH peak is an accurate method to determine the ovulation time.

III. Detection of Pregnancy

Witness® Relaxin Pregnancy Test Kit: This is a test kit used to detect pregnancy in dogs and cats. Pregnancy can be detected as early as 20 days after mating.

Serum relaxin level detection: Relaxin is mainly secreted by the placenta and is a reliable indicator of pregnancy. Relaxin levels remain high throughout pregnancy but drop rapidly after delivery or abortion.

In summary, the results of dog breeding detection require comprehensive consideration of multiple aspects, including the judgment of estrus cycle, determination of ovulation time, and detection of pregnancy. Through scientific methods and technological means, a more accurate grasp of dog breeding conditions can be achieved, improving breeding efficiency and success rates.